FIGURE 3.
Characterization of Lpcat1 enzymatic activity for histone H4 protein palmitoylation. In vitro palmitoylation experiments were carried out in the presence of different amounts of Lpcat1 recombinant protein (A), optimized for reaction time (B), ATP concentration (C), pH (D), temperature (E), and cation dependence (Ca2+ and Mg2+) (F). The data represent three independent experiments.