FIGURE 1.
A, schematic of the chimeric protein (GB1)4-MBP-(GB1)4. B, SDS-PAGE (8%) analysis of purified protein. Lane 1, protein marker; lane 2, (GB1)4-MBP-(GB1)4; and lane 3, (GB1)4-preMBP-(GB1)4). C, gel filtration profiles of the proteins on Superdex 200 analytical column. Elution volume for both (GB1)4-MBP-(GB1)4 and (GB1)4-preMBP-(GB1)4 was 13.3 ml. IgG (150 kDa) and ferritin (440 kDa) were used as markers, which eluted at 14.5 ml and 13.4 ml, respectively. The apparent molecular masses from column calibration were 354 and 316 kDa for (GB1)4-MBP-(GB1)4 and (GB1)4-preMBP-(GB1)4, respectively.