TABLE 1.
Mechanical unfolding properties of MBP and preMBP
| Sample | Contour length change of initial peak ΔLM | Contour length change of intermediate ΔLI | Unfolding force of initial peak | Unfolding force of intermediate | % Traces with intermediatea | |
|---|---|---|---|---|---|---|
| nm | nm | pN | pN | |||
| MBP | No ligand | 100 ± 8b (n = 133)c | 50 ± 8 (n = 82) | 77 ± 22 | 45 ± 19 | 61.7 ± 4.3 |
| Maltose (0.2–10 mm) | 100 ± 9 (n = 86) | 50 ± 8 (n = 68) | 76 ± 24 | 43 ± 18 | 79.1 ± 4.5 | |
| Maltotriose (0.5–10 mm) | 102 ± 11(n = 57) | 50 ± 9 (n = 47) | 79 ± 29 | 45 ± 21 | 82.5 ± 5.2 | |
| PreMBP | No ligand | 100 ± 9 (n = 46) | 51 ± 8 (n = 24) | 75 ± 20 | 34 ± 10 | 52.2 ± 7.4 |
| Maltose (0.2–10 mm) | 100 ± 8 (n = 78) | 53 ± 10 (n = 53) | 77 ± 30 | 52 ± 30 | 67.9 ± 5.4 | |
| Maltotriose (0.5–10 mm) | 102 ± 14 (n = 53) | 52 ± 10 (n = 38) | 79 ± 30 | 55 ± 37 | 71.7 ± 6.2 | |
a Percent traces with Intermediate = (No. of traces with the intermediate/total number of traces) × 100. The errors are S.E., calculated using the bootstrap method (49, 50) (see supplemental text).
b All reported values are average ± S.D.
c The number of measurements per experiment is given in parentheses.