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. 2011 Jun 15;286(32):28128–28137. doi: 10.1074/jbc.M111.255794

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Complementation of Pa5196 6248 (dprE1) mutant. A, twitching motility of the Pa5196 wild type and its 6248 mutant complemented with the pBADGr vector control, the cognate gene, or homologues from P. aeruginosa PAO1 (PA1416), M. smegmatis (MSMEG_6382), or M. tuberculosis (Rv3790). B, pilus preparations of each of the strains in A separated with SDS-PAGE and stained with Coomassie Brilliant Blue or probed with antibodies to PilA_IV (anti-pilin) or to M. tuberculosis lipoarabinomannan (anti-LAM). M, molecular mass markers in kDa. Genes that restore twitching motility also restore pilin glycosylation and increase the amount of recoverable surface pili.