FIGURE 5.

Mutations in RORβ binding site abolish Nrl promoter activity. Representative sections from neonatal mouse retina transfected in vivo with the ubiquitin (Ub)-GFP construct (left panels) and Nrl promoter segment −938 to +119 wild type (upper center) and mutant for the RORβ binding site (lower center, ROR site located from −783 to −771) are shown. Retinas were harvested at P14 for examining the expression of the reporter gene. Right panels show merged images. Green, GFP fluorescence; red, mCherry fluorescence; blue, DAPI staining. Scale bar, 20 μm.