FIGURE 3.

DHRS3 is an endoplasmic reticulum protein. A, U2OS cells were transfected with constructs expressing GFP or a DHRS3-GFP fusion protein for 24 h. Calreticulin (Calr) is an ER marker, and DAPI demarcates the nucleus. B, ZR-75-1, MDA-MB-175III, and HepG2 cells were partitioned into total (Tot), cytosolic-enriched (Cyto), or ER-enriched fractions and analyzed by Western blot. Actin is a cytosolic marker; calreticulin is an ER marker. C, a hydropathy plot of human DHRS3. The x axis represents amino acid position and the y axis represents amino acid hydropholicity (negative score) and hydrophobicity (positive score). The predicted endoplasmic reticulum targeting signal (ERTS) is shown. D, U2OS cells were transfected with GFP alone or the indicated DHRS3 fusion constructs for 24 h. The cells were immunostained for calreticulin, an ER control, and stained for DAPI to demarcate the nucleus.