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. 2011 Jun 9;286(32):28343–28356. doi: 10.1074/jbc.M111.254227

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Ectopic DHRS3 expression enhances lipid droplet accumulation. A, U2OS cells were transfected with three different concentrations of GFP or DHRS3-GFP and analyzed for lipid droplet accumulation. LipidTOX Red marks the lipid droplets. B, transmission electron microscopy of immunogold-labeled ectopic DHRS3 in U2OS stable cell lines. U2OS monolayers were fixed, probed with α-DHRS3 primary antibody, and visualized by transmission electron microscopy. Representative lipid droplets (LD) are indicated. Panels 1 and 2 are 25,000× magnification and panels 3 and 4 are 50,000× magnification, respectively.