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. 2011 May 25;286(32):28444–28455. doi: 10.1074/jbc.M111.244517

FIGURE 1.

FIGURE 1.

Detection and siRNA validation of human Letm1 on the mRNA level of Ea.hy926 cells. Panel A, RT-PCR using specific primers for Letm1 mRNA (see “Experimental Procedures”) yielded a clear 530 bp product amplification. Panel B, points of applications of 2 different siRNAs (see “Experimental Procedures”) against Letm1 are illustrated within the open reading frame of the mRNA of Letm1. Efficiency of siRNA-mediated Letm1 knock-down was verified by real time quantitative-PCR after transfection of siRNA1 (si1-Letm1, n = 3) or siRNA2 (si2-Letm2, n = 3) against Letm1 individually or both in combination (si1/si2-Letm1, n = 3) versus Control siRNA (Control, n = 3). Data are expressed in % of the maximal response in Control. *, p = 0.013; **, p = 0.0019; ***, p < 0.0001 versus Control. mRNA expression levels of either UCP2 or UCP3 were not influenced by the knock-down of Letm1 using the sample that was treated with both siRNAs against Letm1.