FIGURE 7.

Overexpression of Letm1 co-localized with mitochondria but did not affect mitochondrial Ca²⁺ uptake from any resources. Plasmids encoding for Letm1-mCherry or Letm1 were transiently transfected in endothelial cells that stably express RP-mt. Panel A, mitochondria were visualized using RP-mt (left panel), Letm1 that was N-terminally tagged to mCherry was imaged to test protein targeting (middle panel). The overlay shows Letm1-mCherry exclusively targeted to the mitochondria (right panel). Panel B, overexpression of Letm1 did not affect mitochondrial sequestration of either intracellularly released or entering Ca²⁺. Cells were co-transfected with Letm1 together with a nuclear-targeted GFP in a ratio 3:1 (Letm1: n = 9, 26 cells) or nuclear-targeted GFP alone (Control: n = 9, 25 cells).