FIGURE 3.

Proteomic analysis of the MARS complex in C. elegans. a, the MARS complex in C. elegans was affinity purified with antibodies directed to the core domain of MetRS-Ce. Bound proteins were eluted, analyzed by SDS-PAGE, and detected by silver stain. Polypeptides identified by LC-MS/MS are indicated. The MARS complex purified from rabbit liver is shown as a control. b, the MetRS component was also identified by Western blotting (WB) using anti-MetRSΔC-Ce antibodies. The polypeptide marked with as asterisk (*) corresponds to a degradation product of MetRS-Ce. c, small proteins were also separated on a 12% polyacrylamide gel, before their analysis by LC-MS/MS. They corresponded to ribosomal proteins (see Table 1).