FIGURE 3.

SMS2 deficiency prevents diet-induced fatty liver formation. A, after the DIO test, surgical examinations of the abdomen and liver of the mice were performed. B, Oil-Red-O staining was performed using frozen sections of liver. Triglyceride levels in liver (C), plasma levels of free fatty acid (E; free fatty acid (FFA)), triglyceride (F; TG), and cholesterol (G) were determined as described under “Experimental Procedures.” D, amount of epididymal WAT was also measured. Data are presented as the mean ± S.D. for nine mice. *, p < 0.05; #, p < 0.01; N.S., not significant.