FIGURE 10.
Effect of curcumin on IL-1β-induced Akt phosphorylation. Serum-starved human tenocytes (1 × 106 cells/ml) were either stimulated with 10 ng/ml of IL-1β for the indicated times or pre-treated with 5 μm curcumin for 4 h and exposed to 10 ng/ml of IL-1β for the indicated times. Whole cell extracts were analyzed by Western blot analysis using anti-phospho-specific Akt (row I). Cell extracts were immunoprecipitated (IP) with anti-IKK-α antibody and the immunoprecipitates were separated (500 ng of protein/lane) by SDS-PAGE and analyzed by immunoblotting (IB) using anti-Akt antibody (row II) or with anti-IKK-α antibody (row III, as a loading control). Results shown are representative of three independent experiments.