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. 2011 Jun 20;286(32):28574–28583. doi: 10.1074/jbc.M111.227942

FIGURE 5.

FIGURE 5.

RIG-I CARD2 negatively regulates MDP-induced NOD2 inflammatory signaling. A, luciferase assay (expressed as fold NF-κB induction versus vector controls) from HEK293 cells transfected with HA-NOD2 (300 ng) and either vector or EGFP-RIG-I CARD1 or CARD2 (300 ng) and an NF-κB promoted luciferase construct (100 ng). B, luciferase assay (expressed as fold NF-κB induction versus vector controls) from HEK293 cells transfected with vector or EGFP-RIG-I CARD2 (200 ng) at the indicated concentrations and HA-NOD2 and an NF-κB promoter luciferase construct (100 ng). Transfected cells were exposed to 10 μg/ml MDP for 18 h and luciferase measured. Data are shown as mean ± S.D. Asterisks indicate p values < 0.05 (*) or <0.001 (**). Immunoblots from lysates are shown at the bottom of A and B.