Skip to main content
. 2011 Jun 27;286(32):28608–28618. doi: 10.1074/jbc.M111.225839

FIGURE 2.

FIGURE 2.

Expression and subcellular localization of VAMP2 and VAMP3 in JG cells. A, a representative Western blot shows expression of VAMP2 (18 kDa) and VAMP3 (11 kDa) in a JG cell lysate. The top panel is VAMP2, and the bottom panel is VAMP3. Lane 1 is brain homogenate (2.5 μg) used as a positive control, and lane 2 is JG cell lysate (7.5 μg) (n = 4). B, immunofluorescence and confocal microscopy of VAMP2 and renin on a single mouse JG cell are shown. The left panel shows a representative image from five preparations of a single JG cell labeled with an antibody against renin (green); 32 confocal slices (z-step 0.3 μm) were stacked into one image projection. Large renin-containing granules that range in size from 0.8 to 1.5 μm can be observed. The middle panel shows VAMP2 labeling (red) in the same cell. The right panel shows a merged image illustrating co-localization of renin with VAMP2 as illustrated by a yellow-orange color (n = 5 different preparations). Bar, 3 μm. C, immunofluorescence and confocal microscopy of VAMP3 and renin on a single mouse JG cell are shown. The left panel shows an individual JG cell immunolabeled with renin (green); the middle panel (red) is the same JG cell labeled with VAMP3 antibody; the right panel is the merged image. No co-localization was observed between small VAMP3-labeled vesicles and renin granules.