FIGURE 6.

The density of KDELr particles is reduced by the inhibition of PC and PI synthesis. A, representative images for KDELr staining in NRK and CHO-K1 cells in the presence or absence of choline and inositol. Bar, 10 μm. B, NRK and CHO-K1 cells were incubated in HEPES buffer for 1 h with or without 200 μm choline and 5 mm inositol in the presence of 5 mm LiCl. C, CHO-K1 and CHO-MT58 cells were incubated for 1 h in growth medium at permissive (33 °C) or at non-permissive temperature (40 °C) for the PC-synthesis in CHO-MT58. Results are mean ± S.E. (error bars) of three experiments performed in duplicate.