FIGURE 7.

The density of KDELr particles correlates with the DAG content. Shown are DAG levels (A) and density of KDELr particles (B) from a representative experiment. C, density of KDELr particles plotted against DAG for each different experimental condition tested in CHO-K1 and -MT58 cell lines incubated in HEPES buffer for 1 h. Data from individual experiments were normalized (the value for CHO-K1 cells at 33 °C in the absence of substrates was taken as 100%) and represented are means ± S.E. (error bars) of three experiments performed in duplicate. Data points were fitted by non-linear regression to a one-binding site hyperbola (r = 0.896).