FIGURE 8.

Post-Golgi transport of YFP-ts045VSV-G is altered by the regulation of PC and PI synthesis. CHO-K1 cells transiently transfected with YFP-ts045VSV-G were incubated overnight at 40 °C to accumulate the unfolded YFP-ts045VSV-G at the ER. Cells were then shifted to 32 °C and incubated in HEPES buffer with or without 200 μm choline and 5 mm inositol in the presence of 5 mm LiCl for the indicated times. A, representative images for the two conditions. Bar, 10 μm. B, percentage of cells with YFP-ts045VSV-G at the Golgi for the times indicated. Results are mean ± S.E. of three experiments performed in duplicate. C, cells incubated for 60 min as in B were then labeled with anti-VSV-G ectodomain antibody without permeabilization. Results represent the ratio between the staining of VSV-G at the plasma membrane and the total cellular YFP-ts045VSV-G. Results are mean ± S.E. of five experiments performed in triplicate.