Table 2. Analysis of NS1 ED-monomer homology.

Root-mean-square deviations (r.m.s.d.s) were calculated for each monomer with respect to two archetypes, 2gx9_A and 3d6r_A, using PyMOL. Values in bold indicate the lower r.m.s.d. relationship. Strain abbreviations are as follows: PR8, A/Puerto Rico/8/34; Alb/76, A/Duck/Albany/60/76; Ud/72, A/Udorn/72; VN/04, A/Viet Nam/1203/2004; Cal/04, A/California/07/2009.

Strain	Structure (PDB code_chain)	Strand–strand dimer	2gx9_A r.m.s.d. (Å)	3d6r_A r.m.s.d. (Å)
Wild-type NS1 ED
PR8	2gx9_A	Yes	0.00	0.91
PR8	2gx9_B	Yes	0.30	0.87
PR8	3o9s_A	No	0.85	0.73
PR8	3o9s_B	No	0.85	0.60
PR8	3o9u_A	Yes	0.57	0.72
PR8	3o9u_B	Yes	0.55	0.63
PR8	3o9u_C	Yes	0.56	0.63
PR8	3o9u_D	Yes	0.57	0.72
PR8	3o9u_E	Yes	0.55	0.63
PR8	3o9u_F	Yes	0.55	0.63
PR8	3o9u_G	Yes	0.57	0.63
PR8	3o9u_H	Yes	0.57	0.63
PR8	3o9t_A	No	0.61	0.58
PR8	3o9t_B	No	0.59	0.57
Alb/76	3d6r_A	No	0.91	0.00
Alb/76	3d6r_B	No	0.98	0.31
Alb/76	3oa9_A	No	0.98	0.37
Alb/76	3oa9_B	No	0.81	0.52
Ud/72	3ee9_A	No	0.87	0.52
Ud/72	3ee9_B	No	0.63	0.59
Ud/72	3ee8_A	No	0.87	0.52
Ud/72	3ee8_A	No	0.84	0.53
VN/04	3f5t_A	No	1.04	0.61
Cal/07	3m5r_A	No	0.79	0.46
Cal/07	3m5r_B	No	0.69	0.61
Cal/07	3m5r_D	No	0.75	0.55
Cal/07	3m5r_E	No	0.67	0.56
Cal/07	3m5r_F	No	0.68	0.56
Cal/07	3m5r_G	No	0.71	0.69
Mutant NS1 ED
PR8 (W187A)	3o9r_A	Yes	0.59	0.82
PR8 (W187A)	3o9r_B	Yes	0.56	0.79
PR8 (W187A)	3o9q_A	Yes	0.56	0.66
PR8 (W187A)	3o9q_B	Yes	0.61	0.64
PR8 (W187A)	3rvc_A	Yes	0.49	0.69
Ud/72 (W187A)	3kwg_A	No	0.92	0.49
Ud/72 (W187A)	3kwg_B	No	0.81	0.43
Ud/72 (W187Y)	3kwi_A	No	0.83	0.59
Ud/72 (W187Y)	3kwi_B	No	0.65	0.51
NS1 ED in complex
PR8 (with p85β)	3l4q_A	No	1.04	0.52
PR8 (with p85β)	3l4q_B	No	1.05	0.58
Ud/72 (with CPSF30)	2rhk_A	No	0.91	0.39
Ud/72 (with CPSF30)	2rhk_B	No	0.96	0.47
NMR structures of NS1 domains
Ud/72 (W187R)	2kkz_A	No	0.93	0.82