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. Author manuscript; available in PMC: 2012 Jul 28.
Published in final edited form as: J Phys Chem B. 2011 Jul 7;115(29):9320–9326. doi: 10.1021/jp203743m

Table 2.

Fitting parameters for CaM-AF488-Atto 740.a

Parameter 5,44-CaM 78,117-CaM
low Ca2+ 4 μM
Ca2+ 		100 μM
Ca2+ 		low Ca2+ 4 μM
Ca2+ 		100 μM
Ca2+
Ntot 4.16 7.10 4.78 7.17 7.26 6.14
τd(μs)b 405 381 395 406 382 371
τd-RR (μs)c 389 368 376 366 353 340
τ 1 32 39 41 27 29 27
a 0.36 0.12 0.062 0.22 0.18 0.19
c 0.042 0.040 0.055 0.039 0.040 0.059
b 0.35 0.20 0.14 0.55 0.43 0.39
d 0.041 0.064 0.12 0.10 0.095 0.12
a

Triplet parameters were fixed to values determined for the same dye pair attached to polyproline (see Table 1). Parameters for intramolecular dynamics are defined in eq 3.

b

Transit time for GGG(τ), GGR(τ), and GRG(τ).

c

The transit time for GRR(τ) was allowed to vary independently to account for photobleaching of the acceptor during transit through the probe volume.