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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: J Immunol. 2010 Nov 22;186(1):403–410. doi: 10.4049/jimmunol.1001906

Figure 2. PMN contact and isolated membranes induce CASP-6 activation in macrophages.

Figure 2

(A) Relative CASP-6 activity measured by luminogenic substrate with the activity of unstimulated AM defined as 1. Cell extracts were obtained four hours after the stimulus noted on the x axis. The graph represents the combined results of the five BAL cell preparations shown in panels A, B and C. p< 0.03 for the comparison of 5/1 PMN stimulation and either unstimulated control or when PMN and AM are separated by a filter insert. (B) CASP-6 activity measured by luminescent substrate. THP-1 macrophages were incubated with PMN membranes (PMN mem), 1000 ng/ml LPS or membrane fractions of THP-1 (THP-1 mem). The time post stimulation is indicated on the x axis. 10 μg/ml of Z-VEID-FMK was added as a specific inhibitor for CASP-6. Data are means ± SD. * p < 0.001. Representative data from two independent experiments are shown.