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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: J Immunol. 2010 Nov 22;186(1):403–410. doi: 10.4049/jimmunol.1001906

Figure 4. Co-immunoprecipitation and co-localization of IRAK-M with CASP-6.

Figure 4

(A) Western blot probed with IRAK-M antibody. Protein extract from THP-1 macrophages was subjected to immunoprecipitation with isotype control IgG (lane 1), anti-IRAK-M (lane 2), anti-TRAF6 (lane 3) and anti-CASP-6 (lane 4) antibody, and then the precipitants were resolved on SDS-PAGE. (B) Confocal microscopy of primary monocyte-derived macrophages cultured in the absence (upper panel) or the presence of PMN membranes (lower panel). One hr after PMN membrane addition slide chambers were fixed and slides were incubated with goat anti-IRAK-M or rabbit anti-CASP-6 and probed with the Alexa Fluor® 488 conjugated anti-goat Ig (green) and Alexa Fluor® 568 conjugated anti-rabbit Ig (red). Co-localization can be seen as yellow in the merge view. Nuclei were stained with DAPI (blue). Bar 20μm. All experiments were performed at least three times.