Table 1. Comparative ionic permeabilities via chord conductances at −180 mV.
Test Ion | Conc. (mM) | Figure no. | pHo | Size (ϕ) | Im pA | Gm pS | aP/107 cm s−1 |
---|---|---|---|---|---|---|---|
Cl− | 183 | 4 | 7.5 | 1.0 | 26.7 | 148 | 1.13 |
Br− | 175b | 4 | 7.5 | 0.77 | 41.6 | 231 | 2.29 |
I− | 175 | 4 | 7.5 | 0.81 | 44.1 | 245 | 2.30 |
SCN− | 175c | 7 | 7.5 | 1.0 | 35.2 | 196 | 1.49 |
NO3− | 175 | – | 7.5 | 1.0 | 45.8 | 254 | 1.94 |
SCN− | 30b | 7 | 7.5 | 1.0 | 55.6 | 309 | 13.6 |
NO3− | 30 | – | 7.5 | 1.0 | 39.7 | 220 | 9.72 |
NO2− | 30d | 5 | 7.5 | 1.0 | 52.6 | 416 | 12.9 |
Cl− | 183 | 4 | 5.5 | 1.0 | 105.0 | 583 | 4.45 |
Br− | 175b | 4 | 5.5 | 0.77 | 85.6 | 476 | 4.70 |
I− | 175 | 4 | 5.5 | 0.81 | 90.7 | 504 | 4.74 |
SCN− | 175c | 7 | 5.5 | 1.0 | 49.9 | 277 | 2.11 |
NO3− | 175 | – | 5.5 | 1.0 | 45.8 | 254 | 1.94 |
Formate | 175 | – | 5.5 | 1.0 | 15.9 | 88.4 | 0.671 |
Gluconate | 175 | – | 5.5 | 1.0 | 8.3 | 46.2 | 0.351 |
Acetate | 175 | – | 5.5 | 1.0 | 4.6 | 25.5 | 0.194 |
H2PO4− | 170e | – | 5.5 | 1.0 | 1.2 | 6.9 | 0.054 |
Cl− | 30b | 2 | 5.5 | 1.27 | 30.3 | 168 | 5.83 |
SCN− | 30 | 7 | 5.5 | 1.0 | 42.7 | 237 | 10.5 |
NO3− | 30 | – | 5.5 | 1.0 | 39.7 | 220 | 9.72 |
NO2− | 30d | 5 | 5.5 | 1.0 | 60.2 | 730 | 14.7 |
Formally, Gm] −180=−Im]−180/0.18, and the relevant permeability (e.g., for Cl−) is PCl=(RT/2F2)×(Gm]−180)/[Cl−]i, where the factor 2 represents the standard surface area, 2×10−6 cm2. PCl is normalized (÷ ϕ) for cell surface areas (see values in figure legends)
All buffer solutions contained 8.3 mM residual Cl−, which was included in the total concentration used to calculate permeabilities, except for SCN− and NO3−. See legend to Fig. 4
For thiocyanate and nitrate, only their actual concentrations (175 and 30 mM) were used, assuming simultaneous blockade of chloride. See esp. legend to Fig. 7
Calculations done as if NO2− currents added to Cl− currents appropriate for 153 mM. This would be the superchloride behavior suggested in the text description of Fig. 5
Corrected from 175 mM, due to second dissociation at pKa=7.2