. Author manuscript; available in PMC: 2011 Aug 5.

Published in final edited form as: J Med Chem. 2009 Sep 24;52(18):5619–5625. doi: 10.1021/jm900577k

Table 2.

HPLC and ESI-MS data of purified peptides 1–6

Peptides	HPLC t_R (min)^a		ESI-MS (m/z)
Peptides	System 1	System 2	Calculated	Observed
1	13.23	24.08	[M+3H]³⁺=453.3 [M+4H]⁴⁺=340.2 [M+2H]²⁺=679.4	[M+3H]³⁺=453.3 [M+4H]⁴⁺=340.2 [M+2H]²⁺=679.4
2	13.30	25.38	[M+4H]⁴⁺=340.2 [M+3H]³⁺=453.3 [M+2H]²⁺=679.4	[M+4H]⁴⁺=340.2 [M+3H]³⁺=453.3 [M+2H]²⁺=679.4
3	12.78	24.87	[M+4H]⁴⁺=340.2 [M+3H]³⁺=453.3 [M+2H]²⁺=679.4	[M+4H]⁴⁺=340.2 [M+3H]³⁺=453.2 [M+2H]²⁺=679.4
4	13.49	26.99	[M+4H]⁴⁺=340.2 [M+3H]³⁺=453.3 [M+2H]²⁺=679.4	[M+4H]⁴⁺=340.2 [M+3H]³⁺=453.3 [M+2H]²⁺=679.4
5	9.11	16.32	[M+3H]³⁺=434.6 [M+4H]⁴⁺=326.2	[M+3H]³⁺=434.6 [M+4H]⁴⁺=326.2
6	9.63	17.85	[M+3H]³⁺=434.6 [M+4H]⁴⁺=326.2	[M+3H]³⁺=434.6 [M+4H]⁴⁺=326.2

System 1: Solvent B = MeCN; system 2: Solvent B = MeOH; see the experimental section for details. The final purity of all of the peptides by both methods was greater than 98%.