Figure 1.

Effect of endogenous altered cytokinin content on the protein subunit composition of the thylakoid membranes. Chloroplast proteins were solubilized in 1% digitonin and separated in a first native gel electrophoresis (BN-PAGE). Protein subunits were released from protein complexes and were resolved by SDS-PAGE in a second dimension (SDS-PAGE). (A) Average 2D-BN/SDS-PAGE map of Nicotiana tabacum thylakoid membranes. The different protein complexes are indicated above the gel. Identified proteins are indicated with a number and an overview of identified proteins can be found in table 1 and in Additional file 1, table S1. (B-C) 2D-BN/SDS-PAGE map of Pssu-ipt (B) and 35S:CKX1 (C) and corresponding wild-type (WT). (PSI: photosystem I; PSII: photosystem II; LHC: light-harvesting complex; Rubisco: ribulose-bisphosphate carboxylase; cyt b₆f: cytochrome b₆f; LHCII₃: trimeric light-harvesting complex).