Table 1.
Proteins identified by MALDI-TOF/TOF from the second dimension BN/SDS-PAGE.
| Spot number | Protein Name | SwissProt Accession number | MW | pI |
|---|---|---|---|---|
| 1 | ATP synthase subunit alpha, chloroplastic [Nicotiana tabacum] | P00823 | 55477,1 | 5,14 |
| 2 | Photosystem II CP47 chlorophyll apoprotein [Cucumis sativus] | Q2QD63 | 55982,37 | 6,28 |
| 3 | Photosystem II CP43 chlorophyll apoprotein [Ranunculus macranthus] | Q4FFN5 | 52017,79 | 6,68 |
| 4 | Oxygen-evolving enhancer protein 1, [chloroplastic Nicotiana tabacum] | Q40459 | 35377,09 | 5,89 |
| 5 | Chlorophyll a-b binding protein 40, chloroplastic [Nicotiana tabacum] | P27495 | 28450,22 | 5,48 |
| 6 | Chlorophyll a-b binding protein 13, chloroplastic [Solanum lycopersicum] | P27489 | 28661,44 | 5,09 |
| 7 | Chlorophyll a-b binding protein 6A, chloroplastic [Solanum lycopersicum] | P12360 | 26785,52 | 5,82 |
| 8 | Photosystem I reaction center subunit II, chloroplastic [Nicotiana sylvestris] | P29302 | 22466,7 | 9,78 |
| 9 | Photosystem I reaction center subunit II, chloroplastic [Nicotiana sylvestris] | P29302 | 22466,7 | 9,78 |
| 10 | Photosystem I reaction center subunit III, chloroplastic [Spinacia oleracea] | P12355 | 25567,52 | 9,4 |
| 11 | Photosystem II CP43 chlorophyll apoprotein [Aethionema grandiflora] | A4QJJ5 | 52042,77 | 6,71 |
| 12 | Oxygen-evolving enhancer protein 1, chloroplastic [Nicotiana tabacum] | Q40459 | 35377,09 | 5,89 |
| 13 | Ribulose bisphosphate carboxylase large chain (Fragment) [Nelumbo lutea] | Q05800 | 44231,36 | 6,4 |
| 14 | Photosystem I P700 chlorophyll a apoprotein A1 [Nicotiana tomentosiformis] | Q33C36 | 83206,56 | 6,67 |
| 15 | Photosystem II CP43 chlorophyll apoprotein [Ranunculus macranthus] | Q4FFN5 | 52017,79 | 6,68 |
| 16 | Oxygen-evolving enhancer protein 1, chloroplastic [Nicotiana tabacum] | Q40459 | 35377,09 | 5,89 |
| 17 | Photosystem II D2 protein [Dioscorea elephantipes] | A6MMK2 | 39765,89 | 5,34 |
| 18 | Chlorophyll a-b binding protein 40, chloroplastic [Nicotiana tabacum] | P27495 | 28450,22 | 5,48 |
| 19 | Chlorophyll a-b binding protein 6A, chloroplastic [Solanum lycopersicum] | P12360 | 26785,52 | 5,82 |
| 20 | Photosystem I reaction center subunit II, chloroplastic [Nicotiana sylvestris] | P29302 | 22466,7 | 9,78 |
| 21 | Photosystem I reaction center subunit II, chloroplastic [Nicotiana sylvestris] | P29302 | 22466,7 | 9,78 |
| 22 | Photosystem I reaction center subunit IV B, chloroplastic [Nicotiana sylvestris] | Q41229 | 15214,74 | 9,74 |
| 23 | Photosystem I reaction center subunit III, chloroplastic [Flaveria trinervia] | P46486 | 25366,48 | 9,35 |
| 24 | Cytochrome b559 subunit alpha [Arabidopsis thaliana] | P56779 | 9380,7 | 4,83 |
| 25 | ATP synthase subunit alpha, chloroplastic [Nicotiana tabacum] | P00823 | 55477,1 | 5,14 |
| 26 | Ribulose bisphosphate carboxylase large chain [Nicotiana sylvestris] | Q3C1J4 | 53377,98 | 6,41 |
| 27 | Ribulose bisphosphate carboxylase large chain (Fragment) [Adoxa moschatellina] | P28378 | 52159,14 | 6,13 |
| 28 | Photosystem II CP47 chlorophyll apoprotein [Barbarea verna] | A4QKD1 | 56204,47 | 6,4 |
| 30 | Photosystem Q(B) protein [Leptosira terrestri] | A6YGB8 | 38353,08 | 5,52 |
| 31 | Photosystem Q(B) protein [Leptosira terrestris] | A6YGB8 | 38353,08 | 5,52 |
| 32 | Cytochrome b559 subunit alpha [Arabidopsis thaliana] | P56779 | 9380,7 | 4,83 |
| 33 | Apocytochrome f [Nicotiana tabacum] | P06449 | 35337,77 | 9,12 |
| 35 | Cytochrome b6-f complex iron-sulfur subunit 2, chloroplastic [Nicotiana tabacum] | Q02585 | 24491,28 | 8,15 |
| 36 | Cytochrome b6-f complex subunit 4 [Agrostis stolonifera] | A1EA39 | 17534,51 | 6,56 |
| 37 | ATP synthase subunit b, chloroplastic [Nicotiana tabacum] | yP06290 | 20917,96 | 8,76 |
| 38 | Ferredoxin--NADP reductase, leaf-type isozyme, chloroplastic [Nicotiana tabacum] | O04977 | 40704,59 | 8,37 |
| 39 | Fructose-bisphosphate aldolase, chloroplastic [Spinacia oleracea] | P16096 | 42726,8 | 6,85 |
| 40 | Photosystem II 22 kDa protein, chloroplastic [Nicotiana tabacum] | Q9SMB4 | 29068,73 | 6,04 |
| 41 | Apocytochrome f [Nicotiana tabacum] | P06449 | 35337,77 | 9,12 |
| 42 | Photosystem II 22 kDa protein, chloroplastic [Nicotiana tabacum] | Q9SMB4 | 29068,73 | 6,04 |
| 43 | Photosystem II CP43 chlorophyll apoprotein [Aethionema grandiflora] | A4QJJ5 | 52042,77 | 6,71 |
| 44 | ATP synthase subunit beta, chloroplastic [Nicotiana plumbaginifolia] | P69370 | 53548,81 | 5,09 |
| 45 | ATP synthase subunit alpha, chloroplastic [Nicotiana tabacum] | P00823 | 55477,1 | 5,14 |
| 46 | Chlorophyll a-b binding protein CP26, chloroplastic [Arabidopsis thaliana] | Q9XF89 | 30194,7 | 6 |
| 47 | Ferredoxin--NADP reductase, leaf-type isozyme, chloroplastic [Nicotiana tabacum] | O04977 | 40704,59 | 8,37 |
| 48 | ATP synthase subunit alpha, chloroplastic [Nicotiana tabacum] | P00823 | 55477,1 | 5,14 |
| 49 | ATP synthase subunit alpha, chloroplastic [Nicotiana tabacum] | P00823 | 55477,1 | 5,14 |
| 50 | ATP synthase gamma chain, chloroplastic [Nicotiana tabacum] | P29790 | 41705,96 | 8,16 |
| 51 | ATP synthase subunit beta, chloroplastic [Nicotiana tabacum] | P00826 | 53577,78 | 5 |
| 52 | Oxygen-evolving enhancer protein 1, chloroplastic [Nicotiana tabacum] | Q40459 | 35377,09 | 5,89 |
Additional information about the identified proteins can be found in Additional file 1, table S1.