Table 2.
Summary of proteins different in spot abundance ratio between wild-type and transgenic tobacco plants in stroma fraction.
| Stroma fraction Pssu-ipt versus wild-type (SR1) | |||||
|---|---|---|---|---|---|
| Spot number | Protein name + SwissProt accession number | MW | pI | Fold change | p-value |
| 1 | Not identified | - | - | 1.76 | 0.012 |
| 2 | Not identified | - | - | 1.71 | 0.047 |
| 3 | ATP-dependent Clp protease ATP-binding subunit clpA homolog CD4B, chloroplastic [Solanum lycopersicum] P31542 | 102462.8 | 5.86 | 1.98 | 0.039 |
| 4 | Not identified | - | - | -1.44 | 0.023 |
| 5 | Not identified | - | - | 4.47 | 0.025 |
| 6 | Probable fructose-bisphosphate aldolase 1, chloroplastic [Arabidopsis thaliana] Q9SJU4 | 43074.98 | 6.18 | 2 | 0.0114 |
| 7 | Not identified | - | - | 1.91 | 0.0114 |
| 8 | Ferredoxin--NADP reductase, leaf-type isozyme, chloroplastic [Nicotiana tabacum] O04977 | 40704.59 | 8.37 | 1.8 | 0.0134 |
| 9 | Not identified | - | - | 2.18 | 0.046 |
| 10 | Not identified | - | - | 1.99 | 0.0114 |
| 11 | Not identified | - | - | 1.9 | 0.039 |
| 12 | Not identified | - | - | 2.23 | 0.043 |
| 13 | Oxygen-evolving enhancer protein 2-1, chloroplastic [Nicotiana tabacum] Q7DM39 | 28805.44 | 6.84 | 3.02 | 9.40E-03 |
| 14 | Superoxide dismutase [Fe], chloroplastic (Fragment) [Nicotiana plumbaginifolia] P22302 | 23027.52 | 5.53 | 4.84 | 6.70E-03 |
| 15 | Not identified | - | - | 2.97 | 6.7E-03 |
| 16 | Not identified | - | - | 2.37 | 0.0146 |
| 17 | Not identified | - | - | 1.96 | 0.0146 |
| 18 | Not identified | - | - | 1.48 | 0.0146 |
| 19 | Not identified | - | - | -1.57 | 6.7E-03 |
| Stroma fraction 35S:CKX1 versus wild-type (Samsun NN) | |||||
| Spot number | Protein name + SwissProt accession number | MW | pI | Fold change | p-value |
| 6 | Probable fructose-bisphosphate aldolase 1, chloroplastic [Arabidopsis thaliana] Q9SJU4 | 43074.98 | 6.18 | 1.42 | 5.46E-03 |
| 17 | Not identified | - | - | -1.46 | 5.46E-03 |
| 20 | Not identified | - | - | -1.45 | 5.46E-03 |
| 21 | Not identified | - | - | 1.45 | 0.0103 |
| 22 | Not identified | - | - | 1.24 | 9.43E-03 |
| 23 | Probable fructose-bisphosphate aldolase 1, chloroplastic [Arabidopsis thaliana] Q9SJU4 | 43074.98 | 6.18 | 1.36 | 5.46E-03 |
| 24 | Probable fructose-bisphosphate aldolase 1, chloroplastic [Arabidopsis thaliana] Q9SJU4 | 43074.98 | 6.18 | 1.39 | 5.46E-03 |
| 25 | Not identified | - | - | 1.3 | 9.43E-03 |
| 26 | Not identified | - | - | -1.66 | 9.43E-03 |
Proteins were identified by MALDI-TOF/TOF. The fold change indicates the difference in expression relative to wild-type plants. Additional information about the identified proteins can be found in Additional file 2, table S2.