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IAs/PLP 139-151 dextramers bind rested antigen-specific cells better than tetramers. PLP-reactive cells were generated from SJL mice immunized with PLP 139-151, and were rested in growth medium for 10 days. Viable cells were harvested and stained with PLP 139-151 tetramers or dextramers after being treated with or without NASE. After washing, cells were stained with anti-CD4 and 7-AAD and acquired by FC. Percentages of tet+ or dext+ live (7-AAD-) CD4 cells were then enumerated.
