Table 1. Primers Used for SYBR Green Quantitative RT-PCR Assays.

Gene	Accession Number	Forward Primer (5′ – 3′)	Reverse Primer (5′- 3′)	Amplicon Size (bp)	Primer Spans Exon Junction
TLR1	NM_030682.1	CCGTGATGCACAGCTCCTTGGTT	GGGTATAGGACGTTTCTGTAGGGGT	109	Yes - Reverse
TLR2	NM_011905.3	CTAGGCTGGTGCCCAGATGGCTAG	CCTGCCCGGAGCCTAGGAGTC	70	Yes - Reverse
TLR3	NM_126166.4	TAAAGAGTTCTCCCCGGGGTGTTTCC	GGTGGGGGTTCAGTTGGGCG	83	Yes- Forward
TRR4	NM_021297.2	CCCTGCATAGAGGTAGTTCC	GCCATGCCATGCCTTGTCTTCA	219	Yes - Forward
TLR6	NM_011604.3	ACCGTCAGTGCTGGAAATAGAGCTT	AGGCCAGGGCGCAAACAAAGT	142	Yes - Forward
TLR7	NM_133211.3	AGGCTCTGCGAGTCTCGGTT	GGAGCCAAGGACATCTTTCTTGAAGGC	99	Yes - Reverse
TLR8	NM_133212.2	GAACATGGAAAACATGCCCCTCA	TGCAATCACAAGGGAGTTGTGCCT	145	Yes - Forward
TLR9	NM_031178.2	CTGAGAGACCCTGGTGTGGAACA	CGACGGAGAACCATGTTGGGAGA	96	Yes - Reverse
NLRP3	NM_145827.3	TGCCTGTTCTTCCAGACTGGTGA	CACAGCACCCTCATGCCCGG	144	Yes - Forward
GAPDH	NM_008084.2	ACCCAGAAGACTGTGGATGG	CACATTGGGGGTAGGAACAC	170	No