Table 1. Features of novel introns inserted in MC2R and MC5R genes from a group of ray-finned fishes.
| Fishes | Gene | Intron# | Intron | Flanking | Percentage | Repetitive elements€ | ||
| size | sequences*, @ | GC content | Type (Class) [Diection, d/c$] | From | To | |||
| T. rubripes | MC5R | 41a | 100 | A G AG↑GTCT | 37 | N.A. | N.A. | N.A. |
| T. nigroviridis | MC5R | 41a | 87 | A G AG↑GTAA | 55.2 | N.A. | N.A. | N.A. |
| G. aculeatus | MC5R | 41a | 714 | C G AG↑GTAT | 34.5 | N.A. | N.A. | N.A. |
| O. latipes | MC5R | 41a | 1527 | G G AG↑GTCT | 47.5 | SINE_FR1D (NonLTR/SINE) [d] | 1157 | 1243 |
| T. rubripes | MC5R | 77c | 669 | GCAG↑GTGA | 41.3 | N.A. | N.A. | N.A. |
| T. nigroviridis | MC5R | 77c | 973 | GCAG↑GTGA | 41.1 | (TG)n (Simple repeat) [d] | 301 | 360 |
| G. aculeatus | MC5R | 77c | 1294 | GCAG↑GTAA | 41.4 | UnaL2 (NonLTR/L2) [d] | 2858 | 2929 |
| O. latipes | MC5R | 77c | 4500 | GCAG↑GTGA | 38.5 | Crack-1_HM (NonLTR/Crack) [d] | 31 | 108 |
| DNA-8-23_DR (DNA) [c] | 457 | 552 | ||||||
| MRE1_OL (Interspersed repeat) [d] | 942 | 997 | ||||||
| FUROUSHA2 (DNA/hAT) [d] | 1416 | 1530 | ||||||
| DNA-8-14_DR (DNA) [c] | 1675 | 1778 | ||||||
| FUROUSHA2 (DNA/hAT) [d] | 2056 | 2121 | ||||||
| FUROUSHA2 (DNA/hAT) [d] | 2187 | 2299 | ||||||
| ERV46_MD_I (ERV/ERV1) [d] | 2526 | 2578 | ||||||
| Polinton-1_DR (DNA/Polinton) [d] | 2871 | 2945 | ||||||
| CR1-57_HM (NonLTR/CR1) [c] | 3331 | 3393 | ||||||
| piggyBAC-N1_OL (DNA/piggyBac) [d] | 4080 | 4285 | ||||||
| T. rubripes | MC5R | 140c | 1139 | ACAG↑GTAA | 40 | N.A. | N.A. | N.A. |
| T. nigroviridis | MC5R | 140c | 111 | ACAG↑GTGA | 55.9 | N.A. | N.A. | N.A. |
| G. aculeatus | MC5R | 140c | 302 | ACAG↑GTAA | 38.7 | DNA-8-14_DR (DNA) [c] | 176 | 236 |
| O. latipes | MC5R | 140c | 1963 | ACAG↑GTAA | 40.2 | SINE_FR2 (NonLTR/SINE) [d] | 434 | 795 |
| hAT-2_NV (DNA/hAT) [d] | 1836 | 1883 | ||||||
| T. rubripes | MC2R | 140c | 120 | ACAG↑GTTT | 40 | N.A. | N.A. | N.A. |
| T. nigroviridis | MC2R | 140c | 116 | ACAG↑GTAT | 44 | N.A. | N.A. | N.A. |
| G. aculeatus | MC2R | 140c | 1409 | AC C G↑GTAC | 37.6 | UnaL2 (NonLTR/L2) [c] | 554 | 599 |
| Chapaev-12_HM (DNA/Chapaev) [c] | 634 | 683 | ||||||
| hAT-14_HM (DNA/hAT) [c] | 914 | 1005 | ||||||
| O. latipes | MC2R | 140c | 1857 | AT C G↑GTAC | 43.5 | RTE-1_TD (NonLTR/RTE) [d] | 220 | 297 |
| DNA-TA-5_DR (DNA) [c] | 668 | 789 | ||||||
| RTE-2_AFC (NonLTR/RTE) [d] | 834 | 1201 | ||||||
| RTE-2_AFC (NonLTR/RTE) [d] | 1213 | 1698 | ||||||
| T. rubripes | MC2R | 230c% | 18 | G GC G↑GTGG | 66.7 | N.A. | N.A. | N.A. |
| G. aculeatus | MC2R | 236a | 33 | T GGT ↑GGCC | 93.9 | (CGG)n (Simple repeat) [d] | 4 | 33 |
*The intron insertion points are indicated by ↑ sign.
@
Bases deviating from the proto-splice site sequence (MAG↑R), where M = A/C and R = A/G [48], are marked in italics.
#
Numbering of human MC5 receptor is followed as standard MC receptor.
%
225c-FRU corresponding numbering according to MC2R from T. rubripes.
€
Repetitive elements were predicted using Repeat Masker package, version 3.2.6 and RepBase Censor (http://www.girinst.org/censor/index.php) at default settings.
$Direction - d - direct or c – complementary.