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| Position 1 | Position 2 | Position 3 | ||
| Arg (R) | ||||
| Asp (D) | G-A-[CT] | A-G-[AG]C-G-[ACGT] | T-A-[CT] | Tyr (Y) |
| Glu (E) | G-A-[AG] | |||
| His (H) | C-A-[TC] | T-T-[CT] | Phy (F) | |
| Leu (L) | C-T-[ACGT] | |||
| Ile (I) | A-C-[ACT] | A-A-[CT] | Asn (N) | |
| Ser (S) | A-G-[CT]T-C-[ACGT] | |||
| A-G-[CT]C-G-[ACGT] | Ser (S) | |||
Italics marked nucleotides are standard nucleotide substitution allowed at proto-splice site.
