Figure 5. Twitch and tetanus in EDL muscles before and after creatine treatment.

A, twitch to tetanus ratios expressed relative to the pre-fatigue twitch to tetanus ratio. Before creatine incubation, the twitch to tetanus ratio in fatigued muscle was similar to that in the pre-fatigue state. After creatine incubation however, the twitch to tetanus ratio in the fatigued state was significantly higher than in the pre-fatigue state. Statistically significant differences are indicated by “*” (P<0.05). B, force tracings of twitches and 100-Hz tetani obtained from one EDL muscle. In the fatigued muscle, creatine incubation increases the twitch force and tetanic force. In the recovered muscle however, creatine incubation makes little difference to the twitch and tetanic forces.