Figure 2. Reorganization of transiently transfected rat embryonic fibroblasts (REF52wt) upon mechanical stimulation.

Time series of (A) EGFP-Lifeact and (B) EYFP-actin expressing cells subjected to 4 s⁻¹, 8% unaxial cyclic tensile strain. The double arrow indicates the stretch direction (Scale bars: 25 µm). EGFP-Lifeact cells reorganize their actin stress fiber in a perpedicular direction over time. Stress fibers in EYFP-actin cells exhibit a different response by fusing thicker bundles which get partly brigded by other fibers. See supplemntary movies (S1, S2) for further details of the reorganization processes.