Skip to main content
. 2011 Aug 5;6(8):e23266. doi: 10.1371/journal.pone.0023266

Figure 4. Immunocytochemistry of hESC cultures for SSEA-4 expression and binding to lectins.

Figure 4

The panels in the left column show hESCs staining for SSEA-4 and DAPI (panels A, D, G, J, M, P, S, and V). In the same field of view as the left panel, the middle panels show binding of ConA(B), Pha-L (E), MMA (H), VVA (K), DBA (N), Pha-E (Q), LTL (T), and PNA (W) lectins in hESCs. The merge view of lectin and SSEA-4 staining is shown in panels in the right column. The staining amount roughly correlates with flow cytometry analysis. Scale bar: 10 µm.