Figure 1. to mRNA levels are increased in slob^IP1 fly heads but still cycle.

A, Western blot demonstrating rescue of SLOB expression in fly heads using two separate drivers for mNSCs in the slob^IP1 background. B , C , to mRNA levels in fly heads were measured by qPCR. to relative transcript levels are increased in slob^IP1 flies and rescued by targeted expression of slob in mNSCs. D , to transcript levels cycle in WT^P41 and slob^IP1 fly heads. Zeitgeber time (ZT) is plotted on the X axis; the white and black bars represent “lights on” and “lights off”, respectively. Each graph is a summary of a minimum of three independent experiments (mean ± SEM). For comparisons between fly lines, * indicates p<0.05, ** indicates p<0.01, *** indicates p<0.001; for comparisons between ZT points within one fly line, ## indicates p<0.01, One-way ANOVA with Bonferroni post-test.