Figure 2. TO protein levels are increased in slob^IP1 fly heads but still cycle.

TO protein levels in fly heads were measured by Western blot analysis and normalized to MAPK levels. A , Representative Western blot showing an increase in TO expression in slob^IP1 fly heads compared to WT^P41 fly heads. TO levels are rescued in slob^IP1,mai301>slob fly heads. The graph is a summary of four independent experiments (mean ± SEM). * indicates p<0.05, One-way ANOVA with Bonferroni post-test. B , Representative Western blot showing that TO levels cycle in slob^IP1 fly heads. Zeitgeber time (ZT) is plotted on the X axis; the white and black bars represent “lights on” and “lights off”, respectively. The graph is a summary of three independent experiments (mean ± SEM).