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. 2011 Aug 5;6(8):e23343. doi: 10.1371/journal.pone.0023343

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Sheldon et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Relative dilp2, -3, and -5 transcript levels in fly heads were measured by qPCR. A, B , Relative dilp2 or dilp5 transcript levels are unchanged in slob^IP1 fly heads. C , Relative dilp3 transcript levels are reduced in slob^IP1 fly heads and rescued by expression of SLOB in mNSCs, but not by mutation of to. D, Relative dilp3 transcript levels are increased in heads of flies overexpressing SLOB in mNSCs. E, The increase in to levels is abolished in slob^IP1,dilp3 fly heads. Each graph is a summary of a minimum of three independent experiments (mean ± SEM). *** indicates p<0.001, One-way ANOVA with Bonferroni post-test (C, E) or Student's t-test (D).