Figure 6. slob^IP1 flies exhibit alterations in energy metabolism and insulin pathway signaling.

A , Hemolymph was extracted from flies after fasting, and circulating trehalose and glucose were measured. Slob^IP1 flies display significantly decreased levels of circulating trehalose plus glucose in hemolymph. Results are a summary of a minimum of five independent experiments (mean ± SEM). ** indicates p<0.01, Student's t-test. B , P-AKT levels were measured by Western blot and normalized to MAPK. Representative Western blot showing an increase in P-AKT in slob^IP1 fly heads compared to WT^P41 fly heads. The graph is a summary of eight independent experiments (mean ± SEM). * indicates p<0.05, One sample t-test. C , Whole body trehalose plus glucose levels were measured in flies after fasting. Stored trehalose plus glucose is decreased in slob^IP1 flies and rescued by expression of SLOB in mNSCs. D , Whole body trehalose plus glucose levels are restored in fly lines with decreased levels of to compared to slob^IP1 flies, including dilp3 fly lines. Each stored trehalose graph is a summary of a minimum of six samples (mean ± SEM). * indicates p<0.05, *** indicates p<0.001, One-way ANOVA with Bonferroni post-test. E , Whole body lipid levels were measured and normalized to protein levels. Slob^IP1 flies display increased storage of triglycerides compared to WT^P41 flies, and this effect is rescued by expression of SLOB in mNSCs or by mutation of to in the slob^IP1 background. Results are presented as a summary of a minimum of nine samples (mean ± SEM). * indicates p<0.05, ** indicates p<0.01, One-way ANOVA with Bonferroni post-test.