Figure 7. The effects of SLOB on gene expression and metabolism require SLO.

A, Relative to transcript levels in fly heads were measured by qPCR. WT^P41,slo¹ and slob^IP1,slo¹ express equivalent amount of to in fly heads. B, Whole body trehalose plus glucose levels were measured in flies after fasting. Stored trehalose plus glucose levels are significantly decreased in slob^IP1 flies compared to WT^P41,slo¹ or slob^IP1,slo¹ flies. C, The reduction in dilp3 levels is attenuated in slob^IP1,slo¹ fly heads. Results are a summary of a minimum of three (A, C) or 13 samples (B) (mean ± SEM). * indicates p<0.05, ** indicates p<0.01, *** indicates p<0.001, One-way ANOVA with Bonferroni post-test.