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. 2011 Aug 5;6(8):e23304. doi: 10.1371/journal.pone.0023304

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Kim et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Microphotographs in the left panel (1, 4, 7) show the oocytes under bright field, whereas microphotographs in the middle panel (2, 5, 8) show the noninvasive analysis of spindle structure in same oocytes using Polscope. Microphotographs in the right panel (3, 6, 9) show the results of aceto-orcein staining of chromosomes in MII oocytes. (B) Immunofluorescence staining for spindle and chromosome morphology in control and Gas6 dsRNA-injected oocytes matured in vitro. MII oocytes were fixed in 4% paraformaldehyde, stained with α-tubulin antibody (Green), and counterstained with propidium iodide (Red) for DNA staining. Scale bars indicate 25 µm.