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. 2011 Aug 5;6(8):e23304. doi: 10.1371/journal.pone.0023304

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Kim et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Microphotographs of embryos after parthenogenetic activation. MII oocytes of various groups were activated by SrCl₂ and cultured in CZB medium to observe parthenogenetic development. (a–c) Control oocytes developed to PN and 2C stages, whereas (d) Gas6-silenced oocytes were arrested at the MII stage. Scale bars indicate 100 µm. (B) Developmental stages of parthenogenetically activated oocytes. Gas6-silenced MII oocytes were all arrested at the MII stage and not activated.