Table 4. Primer sequences and RT-PCR conditions used for Gas6 RNAi.
| Gene | Accession numbers | Primer sequence * | Annealing temperature | Product size |
| Gas6-A** | BC005444 | For- CCGTGATTAGACTACGCTTCRev- AGTTGAGCCTGTAGGTAGCA | 60°C | 561 bp |
| Gas6-B** | BC005444 | For- AAAGGGCCAGAGTGAAGTGARev- TTTTCCCGTTTACCTCCAGA | 60°C | 175 bp |
| Gdf9 | NM_008110 | For- GGTTCTATCTGATAGGCGAGGRev- GGGGCTGAAGGAGGGAGG | 65°C | 446 bp |
| Plat | NM_008872 | For- CATGGGCAAGAGTTACACAGRev- CAGAGAAGAATGGAGACGAT | 60°C | 631 bp |
| Mos | NM_020021 | For- TGGCTGTTCCTACTCATTTCRev- CTTTATACACCGAGCCAAAC | 60°C | 273 bp |
| H1foo | NM_138311 | For- GCGAAACCGAAAGAGGTCAGAARev- TGGAGGAGGTCTTGGGAAGTAA | 60°C | 378 bp |
| GFP | EU056363 | For- ATGGTGAGCAAGGGCGAGRev- CTTGTACAGCTCGTCCAT | 60°C | 717 bp |
| Gapdh | BC092294 | For- ACCACAGTCCATGCCATCACRev- TCCACCACCCTGTTGCTGTA | 60°C | 451 bp |
*For = Forward; Rev = Reverse.
**Primer set-A was used for the preparation of dsRNA, whereas set-B was used to confirm the gene-specific knockdown after RNAi.