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GSK-3β inhibition alters the expression of anti-apoptotic proteins. GSK-3β was inhibited in Neuro-2A cells either by treating with a small molecule inhibitor of GSK-3β SB415286 (25 μM) or with a specific shRNA for GSK-3β. Western blot analysis was performed to determine the cellular protein levels of β-catenin, XAIP and Bcl-2. Actin was used to assess protein loading in each lane
