FIG. 6.
Perivascular AngII treatment induced medial SHP-2 dephosphorylation that was reversed by co-treatment with catalase. The perivascular layer of rat carotid arteries was incubated in situ for 90 min with vehicle (n = 4), AngII (500 nM; n = 4), or AngII + catalase (3,000 U/ml; n = 4). Medial homogenates were subjected to Western blot with phospho-SHP-2 (SHP-2-[Tyr542]) or GAPDH antibody. Bar graphs represent averaged optical-density data expressed as a ratio of phospho-SHP2 to GAPDH. *p < 0.05 indicates a significant difference between vehicle and AngII groups. #p < 0.05 indicates a significant difference between AngII and AngII + catalase.