Table 2.
Melting temperatures of curved and normal DNAs in different buffers
DNA | Buffer B | Buffer C | Buffer F | A+T Ratio, % |
||
---|---|---|---|---|---|---|
Left | Right | Average | ||||
Normal 198-bp | 53.8, 55.21 | 58.4, 60.01 | 84.7, 86.51 | 34 | 52 | 42 |
Curved 199-bp | 47.8 | 47.5 | 80.4 | 62 | 54 | 58 |
Normal 208-bp | 53.8, 55.21 | 54.6 | 84.2, 86.11 | 53 | 38 | 44 |
Curved 207-bp | 45.7 | 46.4 | 77.7 | 71 | 61 | 66 |
Two thermal transitions were observed.