Table 1.
Volume | Protein | Total protein | hbFGF | Total hbFGF | Recovery | Purification fold | |
---|---|---|---|---|---|---|---|
(mL) | (mg/mL) | (mg) | (mg/mL) | (mg) | % | ||
Denatured cell lysate | 300 | 4.2 | 1260 | 1.26 | 378 | ||
Flow through | 910 | 0.73 | 664.3 | 0.02 | 23.3 | 6.16 | 0.116 |
Peak 1 | 183 | 2 | 366 | 1.91 | 349.72 | 92.52 | 3.51 |
Peak 2 | 162 | 0.1 | 16.2 | 0.03 | 4.86 | 1.285 | 1.11 |