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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Apr 5;1820(6):675–683. doi: 10.1016/j.bbagen.2011.03.013

Table 1.

Overview of methodologies for detection of SNO-proteins.

Class 1: Detection of intact SNOa
X-ray crystallography
UV-vis spectroscopy
NMR spectrocopy
Mass spectrometry
SNO-specific antibodies
Class 2: Detection of SNO-derived nitrite and NOb
Saville Assay
DAF-2 Assay
GC-MS
Photolysis chemiluminscence
Reductive chemiluminescence
NO electrode
Class 3: Labeling of SNO-derived Cys thiolc
Biotin switch technique (BST)
SNO-site identification (SNO-SID)
S-nitrosothiol capture (SNOCAP)
Resin-assisted capture of SNO-Proteins (SNO-RAC)
Spin trapping after UV photolysis
Organomercurial-binding
Phosphine-based ligation
a

These methods, except for SNO-based antibodies, are mostly suitable for the characterization of purified SNO-proteins and are generally low-sensitivity.

b

These methods are suitable for quantifying total levels of endogenous SNO-proteins but have limited utility for the analysis of specific SNO-proteins from complex mixtures.

c

These methods are useful for the enrichment, identification and relative quantification of SNO-proteins from complex mixtures and for the facile identification of SNO-sites.

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