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. Author manuscript; available in PMC: 2011 Aug 8.
Published in final edited form as: Biochem Biophys Res Commun. 2010 May 10;396(4):915–920. doi: 10.1016/j.bbrc.2010.05.022

Figure 1. Selective expression of PiwiL2 in adult and aged bone marrow MSCs.

Figure 1

(a) RT-PCR quantification of PiwiL2 transcripts: RT-PCR was implemented on RNA from passage 12 bone marrow MSCs from mice at the indicated age groups as described in Methods (n=3; p<0.05). (b) FACS quantification of PiwiL2 expression: MSCs from each age group at passage 14 were analyzed by FACS as described in Methods, results are representative of 3 separate experiments. (c) PiwiL2 expression in testis and MSCs: protein lysates were prepared from mouse testis or MSCs from 8-month mice, immunoprecipitated and analyzed by Western blots as described in Methods. (d) Identification of micro-RNAs: MSCs from 2-month mice were transfected with PiwiL2 cDNA; RNA extracted from 2 months ± transfection or 8 months was analyzed by Northern blot and probed with anti-piRNA oligonucleotide as described in Methods. (e) Northern Blot comparison of testis and MSC piRNA: RNA was purified from mouse testes or MSCs as indicated. Testis (20μg) and MSC (200μg) RNAs were electrophoresed on 15% denaturing PAGE gels and stained with SybrGold (Molecular Probes). Results are representative of at least 3 separate experiments.