Table 1.

Ankyrin consensus sequence and variants used in this study.

1

6

11

16

21

26

31

•

•

•

•

•

•

•

Secondary structurea

e

e

e

e

e

e

H

H

H

H

H

H

H

e

e

e

H

H

H

H

H

H

H

H

H

e

e

e

e

e

e

e

e

N-terminal cap (N)

S

K

D

G

N

T

P

L

H

N

A

A

K

N

G

H

A

E

E

V

K

K

L

L

S

K

G

A

D

V

N

A

R

Consensus repeat (R)

S

K

D

G

N

T

P

L

H

L

A

A

K

N

G

H

A

E

I

V

K

L

L

L

A

K

G

A

D

V

N

A

R

Consensus trp-substituted repeat (W)

S

K

D

G

W

T

P

L

H

L

A

A

K

N

G

H

A

E

I

V

K

L

L

L

A

K

G

A

D

V

N

A

R

C-terminal cap (C)

S

K

D

G

N

T

P

E

H

L

A

K

K

N

G

H

H

E

I

V

K

L

L

D

A

K

G

A

D

V

N

A

R

•

•

•

•

•

•

•

1

6

10

16

21

26

31

Sequence substitutions to capping repeats (N and C) and to introduce a tryptophan at position five (N5W) are shown in bold.

^aThe secondary structure, as determined by TALOS+ (Shen et al., 2009) for the central repeat (R) of NRC, is shown along with the numbering scheme used here (H, helix; e, extended). See Figure S5 for cloning procedures