Table 4.

Parameters from temperature dependence studies of consensus ankyrins at pH 5, 20°C.

	mi,i+1/mi=0	mi,i+1/mi=0.5a	mi,i+1/mi=1	mi,i+1/mi=10	SASA based energiesb
					Desolvation	Conformational
ΔGN	6.86±0.06	7.33±0.06	7.59±0.06	8.35±0.07	8.4±0.1
ΔGR	6.02±0.05	6.50±0.05	6.76±0.06	7.52±0.07	10.9±0.1
ΔGC	8.52±0.06	9.00±0.07	9.27±0.07	10.05±0.11	10.7±0.1
ΔGi,i+1	−10.66±0.11	−11.28±0.10	−11.64±0.11	−12.65±0.12	−12.1±0.1
ΔSi	−41.6±4.9	−41.0±5.2	−40.2±5.4	−38.8±5.9	54±0.6	−121±0.3
ΔSi,i+1	56.0±7.1	55.1±7.4	53.9±7.7	51.9±8.4	125±0.6	−67±0.3
ΔCp;i	−51±71	23±73	60±82	191±95	−113±5.6
ΔCp;i,i+1	−330±102	−420±104	−470±117	−640±135	−379±5.6
mi	1.01±0.01	0.73±0.01	0.57±0.00	0.12±0.00	NA
$m_i^S$	2.1±0.5	1.6±0.3	1.3±0.3	0.3±0.1	NA
$m_i^C$	−50±8	−38±6	−30±5	−6±1	NA
I2	8.11×10−5	8.75×10−5	9.31×10−5	11.70×10−5	NA

Free energies are in kcal•mol⁻¹; entropies and heat capacities are in cal•mol⁻¹•K⁻¹; m-values are in kcal•mol⁻¹•M⁻¹; $m_i^S$ and $m_i^C$ are in cal•mol⁻¹•M⁻¹•K. In each column, the m_i,i+1/m_i ratio has been fixed to the indicated value to better constrain the fit. Confidence intervals are the results of 5,000 iterations of bootstrap analysis as described in Table 4. For population plots based on fitted parameters, see Figure S4.