Fig. 7.

ApoA-I stimulates apoE recycling from glia. A: WT primary glia were conditioned for 48 h in the absence or presence of the indicated concentrations of recombinant human apoA-I. Samples were concentrated 10× and analyzed by nondenaturing PAGE and immunodetection for apoE. B: Cell lysates of primary glia treated with the indicated concentrations of recombinant human apoA-I were separated by SDS-PAGE and immunodetected for ABCA1, LDLR, apoE, LRP, and actin as a loading control. C: Quantitation of cellular ABCA1, LDLR, apoE, and LRP levels in WT glia-treated apoA-I. Data represent means and standard error from three independent experiments using one-way ANOVA with Tukey's multiple comparison post test.